暗夜下的高谭市，迎来新一年的万圣节。偏偏就在这样一个宁静的夜晚，银嗓女妖（Kari Wuhrer 配音）救出所罗门格兰迪，在闹市区繁华街道引发混乱，危机时刻蝙蝠侠（Roger Craig Smith 配音）、绿箭侠（Chris Diamantopoulos 配音）等正义战士出现，暂时化解危机。在此之后，稻草人、泥面人随同前面两个恶棍接连登场，而他们的幕后指使正是诡计多端、邪恶狡猾的小丑（Troy Baker 饰）。此时此刻，小丑正策划邪恶的阴谋，他试图在高谭市传播一种能令人狂笑的电脑病毒，足以使这座城市陷入前所未有的混乱。 蝙蝠侠、绿箭侠以及生化人（Khary Payton 配音）、夜翼（Will Friedle 配音）还有红罗宾（Yuri Lowenthal 配音）出动迎战……
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